| Microorganism name |
Kluyveromyces lactis
|
| Target proteins |
Beta-lactoglobulin (Rocha et al., 1996)
|
| Wild-type or GMO |
GMO (Rocha et al., 1996)
|
| Production mode (intracellular/extracellular) |
Extracellular (Rocha et al., 1996)
|
| Protein yield (g/L or g/g?) |
0,030 g/L (Rocha et al., 1996)
|
| Temperature used in study |
28°C (Rocha et al., 1996)
|
| pH used in study |
NA |
| C & N source |
Glucose, yeast extract, peptone (YPD medium) (Rocha et al., 1996)
|
| Regulatory status in Europe |
Not allowed |
| Regulatory status in other parts of the world |
Has been filed forFDA GRAS approval in the US. Not allowed in Canada
|
| Companies |
Shangai Changing Biotechnology
|
| Publications/references |
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Rocha, T. L., Paterson, G., Crimmins, K., Boyd, A., Sawyer, L., & Fothergill-Gilmore, L. A. (1996). Expression and secretion of recombinant ovine β-lactoglobulin in Saccharomyces cerevisiae and Kluyveromyces lactis. Biochemical Journal, 313(3), 927–932. https://doi.org/10.1042/bj3130927
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Spohner, S. C., Schaum, V., Quitmann, H., & Czermak, P. (2016). Kluyveromyces lactis: An emerging tool in biotechnology. Journal of Biotechnology, 222, 104–116. https://doi.org/10.1016/j.jbiotec.2016.02.023
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Barba, D., Beolchini, F., Del Re, G., Di Giacomo, G., & Veglió, F. (2001). Kinetic analysis of Kluyveromyces lactis fermentation on whey: batch and fed-batch operations. Process Biochemistry, 36(6), 531–536. https://doi.org/10.1016/s0032-9592(00)00242-9
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Gomes, A. M. V., Carmo, T. S., Carvalho, L. S., Bahia, F. M., & Parachin, N. S. (2018). Comparison of Yeasts as Hosts for Recombinant Protein Production. Microorganisms, 6(2), 38. https://doi.org/10.3390/microorganisms6020038
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Van Ooyen, A. J., Dekker, P., Huang, M., Olsthoorn, M. M., Jacobs, D. I., Colussi, P. A., & Taron, C. H. (2006). Heterologous protein production in the yeastKluyveromyces lactis. FEMS Yeast Research, 6(3), 381–392. https://doi.org/10.1111/j.1567-1364.2006.00049.x
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Fonseca, G. G., Heinzle, E., Wittmann, C., & Gombert, A. K. (2008). The yeast Kluyveromyces marxianus and its biotechnological potential. Applied Microbiology and Biotechnology, 79(3), 339–354. https://doi.org/10.1007/s00253-008-1458-6
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